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Grace Bio-Labs adhesive secureseal hybridization chamber
Adhesive Secureseal Hybridization Chamber, supplied by Grace Bio-Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/adhesive+secureseal+hybridization+chamber/adhesive+chamber+hybridization+secureseal/pm41723121-343-1-5
Average 86 stars, based on 1 article reviews
adhesive secureseal hybridization chamber - by Bioz Stars, 2026-09
86/100 stars

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Article Title: The Pdgfd-Pdgfrb axis orchestrates tumor-nerve crosstalk in pancreatic cancer
Article Snippet: Fixed samples were washed with Tris-glycine buffer (0.1M glycine, 0.1M Tris-base in DEPC H2O) and 1X PBS for 5 minutes each before being blocked using 100 mM N-succinimidyl acetate (NHS-acetate, ThermoFisher) in CosMx NHS-acetate buffer for 15 minutes.

Article Title: A technical comparison of spatial transcriptomics platforms across six cancer types.
Article Snippet: The slides were incubated at 50 ̊C overnight for aproximately 19 hours with the gene expression panel ("Xenium Human Multi-Tissue and Cancer Panel", PN-1000626, 10X Genomics, which targets 377 human genes).

Saline:

Article Title: Spatial transcriptomics reveals macrophage domestication by epithelial cells promotes immunotherapy resistance in small cell lung cancer.
Article Snippet: Fixed sampleswere rinsed twicewithTris-glycine buffer (0.1Mglycine, 0.1MTrisbase in DEPC H2O) and once with 1X PBS for 5min each before blocking with 100 mM N-succinimidyl (acetylthio) acetate (NHS-acetate, Thermo Fisher Scientific) in NHS-acetate buffer (0.1M NaP, 0.1% Tween pH 8 in DEPCH2O) for 15min at room temperature. .. The sections were then rinsed with 2X saline sodium citrate (SSC) for 5min and an Adhesive SecureSeal Hybridization Chamber (Grace Bio-Labs) was placed over the tissue. .. Briefly, five-micron tissue sections mounted on VWR Superfrost PlusMicro slides (cat# 48311-703) were baked overnight at 60 °C, followed by preparation for in-situ hybridization (ISH) on the Leica Bond RXm system by deparaffinization and heat-induced epitope retrieval (HIER) at 100 °C for 15min using ER2 epitope retrieval buffer (Leica Biosystems product, Tris/EDTA-based, pH 9.0).

Article Title: Cancer-associated fibroblasts shape the formation of budding cancer cells at the invasive front of human colorectal cancer
Article Snippet: This was followed by a tissue digestion step with 3 ug/ml Proteinase K diluted in ACD Protease Plus at 40 °C for 30 min. Then, following a washing with diethyl pyrocarbonate (DEPC) water, the samples were incubated with fiducial beads (Bangs laboratories) in 2X saline sodium citrate and Tween-20 solution (SSCT solution) at room temperature (RT) for 5 min. .. Sections were rinsed with 2X saline sodium citrate (SSC) for another 5 min and sealed with Adhesive SecureSeal Hybridization Chamber (Grace Bio-Labs). .. Then, the fixed samples were blocked with 100 mM N-succinimidyl S-acetylthioacetate (SATA) (Thermo Fisher Scientific) buffer for 15 min at RT.

Article Title: A technical comparison of spatial transcriptomics platforms across six cancer types
Article Snippet: Fixed samples were rinsed twice with Tris- glycine buffer (0.1 M glycine, 0.1 M Tris-base in DEPC H2O) and once with 1X PBS for 5 min each before blocking with 100 mM N-succinimidyl (acetylthio) acetate (NHS-acetate, Thermo Fisher Scientific) in NHS-acetate buffer (0.1 M NaP, 0.1% Tween pH 8 in DEPC H2O) for 15 min at room temperature. .. The sections were then rinsed with 2X saline sodium citrate (SSC) for 5 min and an Adhesive SecureSeal Hybridization Chamber (Grace Bio-Labs) was placed over the tissue. .. At the Dresden Genome Center Facility, the Xenium slides were processed following the "Xenium In Situ for FFPE- Deparaffinization and Decrosslinking" protocol ( CG000580 Rev C, 10X Genomics).

Article Title: The spatial landscape of infectious mononucleosis tonsils
Article Snippet: Slides were then blocked with 100 mM N-succinimidyl (acetylthio) acetate (NHS-acetate, Thermo Fisher Scientific) in NHS-acetate buffer (0.1 M NaP, 0.1% Tween pH 8 in DEPC H2O) for 15 minutes at room temperature. .. Sections were rinsed with 2x saline sodium citrate (SSC) for 5 minutes and an Adhesive SecureSeal Hybridization Chamber (Grace Bio-Labs) was placed over the tissue. .. NanoString CosMx ISH probes were prepared by incubation at 95 °C for 2 min and placed on ice, before the probe mix was pipetted into the hybridization chamber and hybridization was performed at 37C overnight.

Adhesive:

Article Title: Spatial transcriptomics reveals macrophage domestication by epithelial cells promotes immunotherapy resistance in small cell lung cancer.
Article Snippet: Fixed sampleswere rinsed twicewithTris-glycine buffer (0.1Mglycine, 0.1MTrisbase in DEPC H2O) and once with 1X PBS for 5min each before blocking with 100 mM N-succinimidyl (acetylthio) acetate (NHS-acetate, Thermo Fisher Scientific) in NHS-acetate buffer (0.1M NaP, 0.1% Tween pH 8 in DEPCH2O) for 15min at room temperature. .. The sections were then rinsed with 2X saline sodium citrate (SSC) for 5min and an Adhesive SecureSeal Hybridization Chamber (Grace Bio-Labs) was placed over the tissue. .. Briefly, five-micron tissue sections mounted on VWR Superfrost PlusMicro slides (cat# 48311-703) were baked overnight at 60 °C, followed by preparation for in-situ hybridization (ISH) on the Leica Bond RXm system by deparaffinization and heat-induced epitope retrieval (HIER) at 100 °C for 15min using ER2 epitope retrieval buffer (Leica Biosystems product, Tris/EDTA-based, pH 9.0).

Article Title: Tumor cell villages define the co-dependency of tumor and microenvironment in liver cancer.
Article Snippet: Nature Communications | (2026) 17:1986 11 0.1 U/μL SUPERase•InTM [Thermofisher] in DEPC H2O) was pipetted on the tissue slides. .. An adhesive SecureSeal Hybridization Chamber (Grace Bio-Labs) was placed over the tissue. .. The hybridization chamber was sealed to prevent evaporation, and hybridization was performed at 37 °C for 16–18 h. Tissue sections were rinsed of excess probes in 2× SCT for 1min and washed twice in 50% formamide (VWR) in 2× SSC at 37 °C for 25min, then twice with 2× SSC for 2min at room temperature.

Article Title: Cancer-associated fibroblasts shape the formation of budding cancer cells at the invasive front of human colorectal cancer
Article Snippet: This was followed by a tissue digestion step with 3 ug/ml Proteinase K diluted in ACD Protease Plus at 40 °C for 30 min. Then, following a washing with diethyl pyrocarbonate (DEPC) water, the samples were incubated with fiducial beads (Bangs laboratories) in 2X saline sodium citrate and Tween-20 solution (SSCT solution) at room temperature (RT) for 5 min. .. Sections were rinsed with 2X saline sodium citrate (SSC) for another 5 min and sealed with Adhesive SecureSeal Hybridization Chamber (Grace Bio-Labs). .. Then, the fixed samples were blocked with 100 mM N-succinimidyl S-acetylthioacetate (SATA) (Thermo Fisher Scientific) buffer for 15 min at RT.

Article Title: A technical comparison of spatial transcriptomics platforms across six cancer types
Article Snippet: Fixed samples were rinsed twice with Tris- glycine buffer (0.1 M glycine, 0.1 M Tris-base in DEPC H2O) and once with 1X PBS for 5 min each before blocking with 100 mM N-succinimidyl (acetylthio) acetate (NHS-acetate, Thermo Fisher Scientific) in NHS-acetate buffer (0.1 M NaP, 0.1% Tween pH 8 in DEPC H2O) for 15 min at room temperature. .. The sections were then rinsed with 2X saline sodium citrate (SSC) for 5 min and an Adhesive SecureSeal Hybridization Chamber (Grace Bio-Labs) was placed over the tissue. .. At the Dresden Genome Center Facility, the Xenium slides were processed following the "Xenium In Situ for FFPE- Deparaffinization and Decrosslinking" protocol ( CG000580 Rev C, 10X Genomics).

Article Title: Tumor cell villages define the co-dependency of tumor and microenvironment in liver cancer
Article Snippet: The sections were then rinsed with 2× saline sodium citrate (SSC) for 5 min. NanoString ISH probes were prepared by incubation at 95 °C for 2 min and placed on ice, and the ISH probe mix (1 nM 980 plex ISH probe, 10 nM Attenuation probes, 1× Buffer R, 0.1 U/μL SUPERase•InTM [Thermofisher] in DEPC H 2 O) was pipetted on the tissue slides. .. An adhesive SecureSeal Hybridization Chamber (Grace Bio-Labs) was placed over the tissue. .. The hybridization chamber was sealed to prevent evaporation, and hybridization was performed at 37 °C for 16–18 h. Tissue sections were rinsed of excess probes in 2× SCT for 1 min and washed twice in 50% formamide (VWR) in 2× SSC at 37 °C for 25 min, then twice with 2× SSC for 2 min at room temperature.

Article Title: The spatial landscape of infectious mononucleosis tonsils
Article Snippet: Slides were then blocked with 100 mM N-succinimidyl (acetylthio) acetate (NHS-acetate, Thermo Fisher Scientific) in NHS-acetate buffer (0.1 M NaP, 0.1% Tween pH 8 in DEPC H2O) for 15 minutes at room temperature. .. Sections were rinsed with 2x saline sodium citrate (SSC) for 5 minutes and an Adhesive SecureSeal Hybridization Chamber (Grace Bio-Labs) was placed over the tissue. .. NanoString CosMx ISH probes were prepared by incubation at 95 °C for 2 min and placed on ice, before the probe mix was pipetted into the hybridization chamber and hybridization was performed at 37C overnight.

Hybridization:

Article Title: Spatial transcriptomics reveals macrophage domestication by epithelial cells promotes immunotherapy resistance in small cell lung cancer.
Article Snippet: Fixed sampleswere rinsed twicewithTris-glycine buffer (0.1Mglycine, 0.1MTrisbase in DEPC H2O) and once with 1X PBS for 5min each before blocking with 100 mM N-succinimidyl (acetylthio) acetate (NHS-acetate, Thermo Fisher Scientific) in NHS-acetate buffer (0.1M NaP, 0.1% Tween pH 8 in DEPCH2O) for 15min at room temperature. .. The sections were then rinsed with 2X saline sodium citrate (SSC) for 5min and an Adhesive SecureSeal Hybridization Chamber (Grace Bio-Labs) was placed over the tissue. .. Briefly, five-micron tissue sections mounted on VWR Superfrost PlusMicro slides (cat# 48311-703) were baked overnight at 60 °C, followed by preparation for in-situ hybridization (ISH) on the Leica Bond RXm system by deparaffinization and heat-induced epitope retrieval (HIER) at 100 °C for 15min using ER2 epitope retrieval buffer (Leica Biosystems product, Tris/EDTA-based, pH 9.0).

Article Title: Tumor cell villages define the co-dependency of tumor and microenvironment in liver cancer.
Article Snippet: Nature Communications | (2026) 17:1986 11 0.1 U/μL SUPERase•InTM [Thermofisher] in DEPC H2O) was pipetted on the tissue slides. .. An adhesive SecureSeal Hybridization Chamber (Grace Bio-Labs) was placed over the tissue. .. The hybridization chamber was sealed to prevent evaporation, and hybridization was performed at 37 °C for 16–18 h. Tissue sections were rinsed of excess probes in 2× SCT for 1min and washed twice in 50% formamide (VWR) in 2× SSC at 37 °C for 25min, then twice with 2× SSC for 2min at room temperature.

Article Title: Cancer-associated fibroblasts shape the formation of budding cancer cells at the invasive front of human colorectal cancer
Article Snippet: This was followed by a tissue digestion step with 3 ug/ml Proteinase K diluted in ACD Protease Plus at 40 °C for 30 min. Then, following a washing with diethyl pyrocarbonate (DEPC) water, the samples were incubated with fiducial beads (Bangs laboratories) in 2X saline sodium citrate and Tween-20 solution (SSCT solution) at room temperature (RT) for 5 min. .. Sections were rinsed with 2X saline sodium citrate (SSC) for another 5 min and sealed with Adhesive SecureSeal Hybridization Chamber (Grace Bio-Labs). .. Then, the fixed samples were blocked with 100 mM N-succinimidyl S-acetylthioacetate (SATA) (Thermo Fisher Scientific) buffer for 15 min at RT.

Article Title: A technical comparison of spatial transcriptomics platforms across six cancer types
Article Snippet: Fixed samples were rinsed twice with Tris- glycine buffer (0.1 M glycine, 0.1 M Tris-base in DEPC H2O) and once with 1X PBS for 5 min each before blocking with 100 mM N-succinimidyl (acetylthio) acetate (NHS-acetate, Thermo Fisher Scientific) in NHS-acetate buffer (0.1 M NaP, 0.1% Tween pH 8 in DEPC H2O) for 15 min at room temperature. .. The sections were then rinsed with 2X saline sodium citrate (SSC) for 5 min and an Adhesive SecureSeal Hybridization Chamber (Grace Bio-Labs) was placed over the tissue. .. At the Dresden Genome Center Facility, the Xenium slides were processed following the "Xenium In Situ for FFPE- Deparaffinization and Decrosslinking" protocol ( CG000580 Rev C, 10X Genomics).

Article Title: Tumor cell villages define the co-dependency of tumor and microenvironment in liver cancer
Article Snippet: The sections were then rinsed with 2× saline sodium citrate (SSC) for 5 min. NanoString ISH probes were prepared by incubation at 95 °C for 2 min and placed on ice, and the ISH probe mix (1 nM 980 plex ISH probe, 10 nM Attenuation probes, 1× Buffer R, 0.1 U/μL SUPERase•InTM [Thermofisher] in DEPC H 2 O) was pipetted on the tissue slides. .. An adhesive SecureSeal Hybridization Chamber (Grace Bio-Labs) was placed over the tissue. .. The hybridization chamber was sealed to prevent evaporation, and hybridization was performed at 37 °C for 16–18 h. Tissue sections were rinsed of excess probes in 2× SCT for 1 min and washed twice in 50% formamide (VWR) in 2× SSC at 37 °C for 25 min, then twice with 2× SSC for 2 min at room temperature.

Article Title: The spatial landscape of infectious mononucleosis tonsils
Article Snippet: Slides were then blocked with 100 mM N-succinimidyl (acetylthio) acetate (NHS-acetate, Thermo Fisher Scientific) in NHS-acetate buffer (0.1 M NaP, 0.1% Tween pH 8 in DEPC H2O) for 15 minutes at room temperature. .. Sections were rinsed with 2x saline sodium citrate (SSC) for 5 minutes and an Adhesive SecureSeal Hybridization Chamber (Grace Bio-Labs) was placed over the tissue. .. NanoString CosMx ISH probes were prepared by incubation at 95 °C for 2 min and placed on ice, before the probe mix was pipetted into the hybridization chamber and hybridization was performed at 37C overnight.



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Grace Bio-Labs adhesive secureseal hybridization chamber
Adhesive Secureseal Hybridization Chamber, supplied by Grace Bio-Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/adhesive+secureseal+hybridization+chamber/adhesive+chamber+hybridization+secureseal/pm41723121-343-1-5
Average 86 stars, based on 1 article reviews
adhesive secureseal hybridization chamber - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

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Grace Bio-Labs secureseal adhesive hybridization chamber
Secureseal Adhesive Hybridization Chamber, supplied by Grace Bio-Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/adhesive+secureseal+hybridization+chamber/adhesive+chamber+hybridization+secureseal/pm41238915-457-6-10
Average 86 stars, based on 1 article reviews
secureseal adhesive hybridization chamber - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

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