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Grace Bio-Labs adhesive secureseal hybridization chamber
Adhesive Secureseal Hybridization Chamber, supplied by Grace Bio-Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/adhesive+secureseal+hybridization+chamber/adhesive+chamber+hybridization+secureseal/pm41723121-343-1-5
Average 86 stars, based on 1 article reviews
adhesive secureseal hybridization chamber - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Saline:

Article Title: The Pdgfd-Pdgfrb axis orchestrates tumor-nerve crosstalk in pancreatic cancer
Article Snippet: Fixed samples were washed with Tris-glycine buffer (0.1M glycine, 0.1M Tris-base in DEPC H2O) and 1X PBS for 5 minutes each before being blocked using 100 mM N-succinimidyl acetate (NHS-acetate, ThermoFisher) in CosMx NHS-acetate buffer for 15 minutes. .. Prepared samples were rinsed with 2X saline sodium citrate for 5 minutes, after which an Adhesive SecureSeal Hybridization Chamber (Grace Bio-Labs) was placed on the slide to cover the samples. ..

Article Title: Spatial transcriptomics reveals macrophage domestication by epithelial cells promotes immunotherapy resistance in small cell lung cancer.
Article Snippet: Fixed sampleswere rinsed twicewithTris-glycine buffer (0.1Mglycine, 0.1MTrisbase in DEPC H2O) and once with 1X PBS for 5min each before blocking with 100 mM N-succinimidyl (acetylthio) acetate (NHS-acetate, Thermo Fisher Scientific) in NHS-acetate buffer (0.1M NaP, 0.1% Tween pH 8 in DEPCH2O) for 15min at room temperature. .. The sections were then rinsed with 2X saline sodium citrate (SSC) for 5min and an Adhesive SecureSeal Hybridization Chamber (Grace Bio-Labs) was placed over the tissue. .. NanoString® ISH probes were prepared by incubation at 95 °C for 2min and placed on ice, and the ISH probe mix (1 nM 980 plex ISH probe, 10 nM Attenuation probes, 1 nM SMI-0006 custom, 1X Buffer R, 0.1 U/μL SUPERase•InTM [Thermo Fisher Scientific] inDEPCH2O)was pipetted into the hybridization chamber.

Article Title: Cancer-associated fibroblasts shape the formation of budding cancer cells at the invasive front of human colorectal cancer
Article Snippet: Then, the fixed samples were blocked with 100 mM N-succinimidyl S-acetylthioacetate (SATA) (Thermo Fisher Scientific) buffer for 15 min at RT. .. Sections were rinsed with 2X saline sodium citrate (SSC) for another 5 min and sealed with Adhesive SecureSeal Hybridization Chamber (Grace Bio-Labs). .. The ISH probes were incubated at 95 °C for 2 min and placed on ice, then prepared the ISH probe mix (1 nM 960-plex ISH probe, 10 nM Attenuation probes, 1X Buffer R, 0.1 U/uL SUPERase InTM (Thermo Fisher Scientific)) to be pipetted into the hybridization chamber.

Article Title: A technical comparison of spatial transcriptomics platforms across six cancer types
Article Snippet: Fixed samples were rinsed twice with Tris- glycine buffer (0.1 M glycine, 0.1 M Tris-base in DEPC H2O) and once with 1X PBS for 5 min each before blocking with 100 mM N-succinimidyl (acetylthio) acetate (NHS-acetate, Thermo Fisher Scientific) in NHS-acetate buffer (0.1 M NaP, 0.1% Tween pH 8 in DEPC H2O) for 15 min at room temperature. .. The sections were then rinsed with 2X saline sodium citrate (SSC) for 5 min and an Adhesive SecureSeal Hybridization Chamber (Grace Bio-Labs) was placed over the tissue. .. NanoString ISH probes were prepared by incubation at 95 °C for 2 min and placed on ice, and the ISH probe mix (1 nM 980 plex ISH probe, 10 nM Attenuation probes, 1 nM SMI-0006 custom, 1X Buffer R, 0.1 U/μL SUPERase•InTM [Thermo Fisher Scientific] in DEPC H2O) was pipetted into the hybridization chamber and hybridization was performed at 37 °C overnight.

Article Title: A technical comparison of spatial transcriptomics platforms across six cancer types.
Article Snippet: Fixed samples were rinsed twice with Tris- glycine buffer (0.1 M glycine, 0.1 M Tris-base in DEPC H2O) and once with 1X PBS for 5 min each before blocking with 100 mM N-succinimidyl (acetylthio) acetate (NHS-acetate, Thermo Fisher Scientific) in NHS-acetate buffer (0.1 M NaP, 0.1% Tween pH 8 in DEPC H2O) for 15 min at room temperature. .. The sections were then rinsed with 2X saline sodium citrate (SSC) for 5 min and an Adhesive SecureSeal Hybridization Chamber (Grace Bio-Labs) was placed over the tissue. .. AR TIC LE IN PR ES S NanoString ISH probes were prepared by incubation at 95 °C for 2 min and placed on ice, and the ISH probe mix (1 nM 980 plex ISH probe, 10 nM Attenuation probes, 1 nM SMI-0006 custom, 1X Buffer R, 0.1 U/μL SUPERase•InTM [Thermo Fisher Scientific] in DEPC H2O) was pipetted into the hybridization chamber and hybridization was performed at 37 °C overnight.

Article Title: The spatial landscape of infectious mononucleosis tonsils
Article Snippet: Slides were then blocked with 100 mM N-succinimidyl (acetylthio) acetate (NHS-acetate, Thermo Fisher Scientific) in NHS-acetate buffer (0.1 M NaP, 0.1% Tween pH 8 in DEPC H2O) for 15 minutes at room temperature. .. Sections were rinsed with 2x saline sodium citrate (SSC) for 5 minutes and an Adhesive SecureSeal Hybridization Chamber (Grace Bio-Labs) was placed over the tissue. .. NanoString CosMx ISH probes were prepared by incubation at 95 °C for 2 min and placed on ice, before the probe mix was pipetted into the hybridization chamber and hybridization was performed at 37C overnight.

Adhesive:

Article Title: The Pdgfd-Pdgfrb axis orchestrates tumor-nerve crosstalk in pancreatic cancer
Article Snippet: Fixed samples were washed with Tris-glycine buffer (0.1M glycine, 0.1M Tris-base in DEPC H2O) and 1X PBS for 5 minutes each before being blocked using 100 mM N-succinimidyl acetate (NHS-acetate, ThermoFisher) in CosMx NHS-acetate buffer for 15 minutes. .. Prepared samples were rinsed with 2X saline sodium citrate for 5 minutes, after which an Adhesive SecureSeal Hybridization Chamber (Grace Bio-Labs) was placed on the slide to cover the samples. ..

Article Title: Spatial transcriptomics reveals macrophage domestication by epithelial cells promotes immunotherapy resistance in small cell lung cancer.
Article Snippet: Fixed sampleswere rinsed twicewithTris-glycine buffer (0.1Mglycine, 0.1MTrisbase in DEPC H2O) and once with 1X PBS for 5min each before blocking with 100 mM N-succinimidyl (acetylthio) acetate (NHS-acetate, Thermo Fisher Scientific) in NHS-acetate buffer (0.1M NaP, 0.1% Tween pH 8 in DEPCH2O) for 15min at room temperature. .. The sections were then rinsed with 2X saline sodium citrate (SSC) for 5min and an Adhesive SecureSeal Hybridization Chamber (Grace Bio-Labs) was placed over the tissue. .. NanoString® ISH probes were prepared by incubation at 95 °C for 2min and placed on ice, and the ISH probe mix (1 nM 980 plex ISH probe, 10 nM Attenuation probes, 1 nM SMI-0006 custom, 1X Buffer R, 0.1 U/μL SUPERase•InTM [Thermo Fisher Scientific] inDEPCH2O)was pipetted into the hybridization chamber.

Article Title: Tumor cell villages define the co-dependency of tumor and microenvironment in liver cancer.
Article Snippet: Nature Communications | (2026) 17:1986 11 0.1 U/μL SUPERase•InTM [Thermofisher] in DEPC H2O) was pipetted on the tissue slides. .. An adhesive SecureSeal Hybridization Chamber (Grace Bio-Labs) was placed over the tissue. .. The hybridization chamber was sealed to prevent evaporation, and hybridization was performed at 37 °C for 16–18 h. Tissue sections were rinsed of excess probes in 2× SCT for 1min and washed twice in 50% formamide (VWR) in 2× SSC at 37 °C for 25min, then twice with 2× SSC for 2min at room temperature.

Article Title: Cancer-associated fibroblasts shape the formation of budding cancer cells at the invasive front of human colorectal cancer
Article Snippet: Then, the fixed samples were blocked with 100 mM N-succinimidyl S-acetylthioacetate (SATA) (Thermo Fisher Scientific) buffer for 15 min at RT. .. Sections were rinsed with 2X saline sodium citrate (SSC) for another 5 min and sealed with Adhesive SecureSeal Hybridization Chamber (Grace Bio-Labs). .. The ISH probes were incubated at 95 °C for 2 min and placed on ice, then prepared the ISH probe mix (1 nM 960-plex ISH probe, 10 nM Attenuation probes, 1X Buffer R, 0.1 U/uL SUPERase InTM (Thermo Fisher Scientific)) to be pipetted into the hybridization chamber.

Article Title: A technical comparison of spatial transcriptomics platforms across six cancer types
Article Snippet: Fixed samples were rinsed twice with Tris- glycine buffer (0.1 M glycine, 0.1 M Tris-base in DEPC H2O) and once with 1X PBS for 5 min each before blocking with 100 mM N-succinimidyl (acetylthio) acetate (NHS-acetate, Thermo Fisher Scientific) in NHS-acetate buffer (0.1 M NaP, 0.1% Tween pH 8 in DEPC H2O) for 15 min at room temperature. .. The sections were then rinsed with 2X saline sodium citrate (SSC) for 5 min and an Adhesive SecureSeal Hybridization Chamber (Grace Bio-Labs) was placed over the tissue. .. NanoString ISH probes were prepared by incubation at 95 °C for 2 min and placed on ice, and the ISH probe mix (1 nM 980 plex ISH probe, 10 nM Attenuation probes, 1 nM SMI-0006 custom, 1X Buffer R, 0.1 U/μL SUPERase•InTM [Thermo Fisher Scientific] in DEPC H2O) was pipetted into the hybridization chamber and hybridization was performed at 37 °C overnight.

Article Title: A technical comparison of spatial transcriptomics platforms across six cancer types.
Article Snippet: Fixed samples were rinsed twice with Tris- glycine buffer (0.1 M glycine, 0.1 M Tris-base in DEPC H2O) and once with 1X PBS for 5 min each before blocking with 100 mM N-succinimidyl (acetylthio) acetate (NHS-acetate, Thermo Fisher Scientific) in NHS-acetate buffer (0.1 M NaP, 0.1% Tween pH 8 in DEPC H2O) for 15 min at room temperature. .. The sections were then rinsed with 2X saline sodium citrate (SSC) for 5 min and an Adhesive SecureSeal Hybridization Chamber (Grace Bio-Labs) was placed over the tissue. .. AR TIC LE IN PR ES S NanoString ISH probes were prepared by incubation at 95 °C for 2 min and placed on ice, and the ISH probe mix (1 nM 980 plex ISH probe, 10 nM Attenuation probes, 1 nM SMI-0006 custom, 1X Buffer R, 0.1 U/μL SUPERase•InTM [Thermo Fisher Scientific] in DEPC H2O) was pipetted into the hybridization chamber and hybridization was performed at 37 °C overnight.

Article Title: The spatial landscape of infectious mononucleosis tonsils
Article Snippet: Slides were then blocked with 100 mM N-succinimidyl (acetylthio) acetate (NHS-acetate, Thermo Fisher Scientific) in NHS-acetate buffer (0.1 M NaP, 0.1% Tween pH 8 in DEPC H2O) for 15 minutes at room temperature. .. Sections were rinsed with 2x saline sodium citrate (SSC) for 5 minutes and an Adhesive SecureSeal Hybridization Chamber (Grace Bio-Labs) was placed over the tissue. .. NanoString CosMx ISH probes were prepared by incubation at 95 °C for 2 min and placed on ice, before the probe mix was pipetted into the hybridization chamber and hybridization was performed at 37C overnight.

Article Title: Tumor cell villages define the co-dependency of tumor and microenvironment in liver cancer
Article Snippet: The sections were then rinsed with 2× saline sodium citrate (SSC) for 5 min. NanoString ISH probes were prepared by incubation at 95 °C for 2 min and placed on ice, and the ISH probe mix (1 nM 980 plex ISH probe, 10 nM Attenuation probes, 1× Buffer R, 0.1 U/μL SUPERase•InTM [Thermofisher] in DEPC H 2 O) was pipetted on the tissue slides. .. An adhesive SecureSeal Hybridization Chamber (Grace Bio-Labs) was placed over the tissue. .. The hybridization chamber was sealed to prevent evaporation, and hybridization was performed at 37 °C for 16–18 h. Tissue sections were rinsed of excess probes in 2× SCT for 1 min and washed twice in 50% formamide (VWR) in 2× SSC at 37 °C for 25 min, then twice with 2× SSC for 2 min at room temperature.

Hybridization:

Article Title: The Pdgfd-Pdgfrb axis orchestrates tumor-nerve crosstalk in pancreatic cancer
Article Snippet: Fixed samples were washed with Tris-glycine buffer (0.1M glycine, 0.1M Tris-base in DEPC H2O) and 1X PBS for 5 minutes each before being blocked using 100 mM N-succinimidyl acetate (NHS-acetate, ThermoFisher) in CosMx NHS-acetate buffer for 15 minutes. .. Prepared samples were rinsed with 2X saline sodium citrate for 5 minutes, after which an Adhesive SecureSeal Hybridization Chamber (Grace Bio-Labs) was placed on the slide to cover the samples. ..

Article Title: Spatial transcriptomics reveals macrophage domestication by epithelial cells promotes immunotherapy resistance in small cell lung cancer.
Article Snippet: Fixed sampleswere rinsed twicewithTris-glycine buffer (0.1Mglycine, 0.1MTrisbase in DEPC H2O) and once with 1X PBS for 5min each before blocking with 100 mM N-succinimidyl (acetylthio) acetate (NHS-acetate, Thermo Fisher Scientific) in NHS-acetate buffer (0.1M NaP, 0.1% Tween pH 8 in DEPCH2O) for 15min at room temperature. .. The sections were then rinsed with 2X saline sodium citrate (SSC) for 5min and an Adhesive SecureSeal Hybridization Chamber (Grace Bio-Labs) was placed over the tissue. .. NanoString® ISH probes were prepared by incubation at 95 °C for 2min and placed on ice, and the ISH probe mix (1 nM 980 plex ISH probe, 10 nM Attenuation probes, 1 nM SMI-0006 custom, 1X Buffer R, 0.1 U/μL SUPERase•InTM [Thermo Fisher Scientific] inDEPCH2O)was pipetted into the hybridization chamber.

Article Title: Tumor cell villages define the co-dependency of tumor and microenvironment in liver cancer.
Article Snippet: Nature Communications | (2026) 17:1986 11 0.1 U/μL SUPERase•InTM [Thermofisher] in DEPC H2O) was pipetted on the tissue slides. .. An adhesive SecureSeal Hybridization Chamber (Grace Bio-Labs) was placed over the tissue. .. The hybridization chamber was sealed to prevent evaporation, and hybridization was performed at 37 °C for 16–18 h. Tissue sections were rinsed of excess probes in 2× SCT for 1min and washed twice in 50% formamide (VWR) in 2× SSC at 37 °C for 25min, then twice with 2× SSC for 2min at room temperature.

Article Title: Cancer-associated fibroblasts shape the formation of budding cancer cells at the invasive front of human colorectal cancer
Article Snippet: Then, the fixed samples were blocked with 100 mM N-succinimidyl S-acetylthioacetate (SATA) (Thermo Fisher Scientific) buffer for 15 min at RT. .. Sections were rinsed with 2X saline sodium citrate (SSC) for another 5 min and sealed with Adhesive SecureSeal Hybridization Chamber (Grace Bio-Labs). .. The ISH probes were incubated at 95 °C for 2 min and placed on ice, then prepared the ISH probe mix (1 nM 960-plex ISH probe, 10 nM Attenuation probes, 1X Buffer R, 0.1 U/uL SUPERase InTM (Thermo Fisher Scientific)) to be pipetted into the hybridization chamber.

Article Title: A technical comparison of spatial transcriptomics platforms across six cancer types
Article Snippet: Fixed samples were rinsed twice with Tris- glycine buffer (0.1 M glycine, 0.1 M Tris-base in DEPC H2O) and once with 1X PBS for 5 min each before blocking with 100 mM N-succinimidyl (acetylthio) acetate (NHS-acetate, Thermo Fisher Scientific) in NHS-acetate buffer (0.1 M NaP, 0.1% Tween pH 8 in DEPC H2O) for 15 min at room temperature. .. The sections were then rinsed with 2X saline sodium citrate (SSC) for 5 min and an Adhesive SecureSeal Hybridization Chamber (Grace Bio-Labs) was placed over the tissue. .. NanoString ISH probes were prepared by incubation at 95 °C for 2 min and placed on ice, and the ISH probe mix (1 nM 980 plex ISH probe, 10 nM Attenuation probes, 1 nM SMI-0006 custom, 1X Buffer R, 0.1 U/μL SUPERase•InTM [Thermo Fisher Scientific] in DEPC H2O) was pipetted into the hybridization chamber and hybridization was performed at 37 °C overnight.

Article Title: A technical comparison of spatial transcriptomics platforms across six cancer types.
Article Snippet: Fixed samples were rinsed twice with Tris- glycine buffer (0.1 M glycine, 0.1 M Tris-base in DEPC H2O) and once with 1X PBS for 5 min each before blocking with 100 mM N-succinimidyl (acetylthio) acetate (NHS-acetate, Thermo Fisher Scientific) in NHS-acetate buffer (0.1 M NaP, 0.1% Tween pH 8 in DEPC H2O) for 15 min at room temperature. .. The sections were then rinsed with 2X saline sodium citrate (SSC) for 5 min and an Adhesive SecureSeal Hybridization Chamber (Grace Bio-Labs) was placed over the tissue. .. AR TIC LE IN PR ES S NanoString ISH probes were prepared by incubation at 95 °C for 2 min and placed on ice, and the ISH probe mix (1 nM 980 plex ISH probe, 10 nM Attenuation probes, 1 nM SMI-0006 custom, 1X Buffer R, 0.1 U/μL SUPERase•InTM [Thermo Fisher Scientific] in DEPC H2O) was pipetted into the hybridization chamber and hybridization was performed at 37 °C overnight.

Article Title: The spatial landscape of infectious mononucleosis tonsils
Article Snippet: Slides were then blocked with 100 mM N-succinimidyl (acetylthio) acetate (NHS-acetate, Thermo Fisher Scientific) in NHS-acetate buffer (0.1 M NaP, 0.1% Tween pH 8 in DEPC H2O) for 15 minutes at room temperature. .. Sections were rinsed with 2x saline sodium citrate (SSC) for 5 minutes and an Adhesive SecureSeal Hybridization Chamber (Grace Bio-Labs) was placed over the tissue. .. NanoString CosMx ISH probes were prepared by incubation at 95 °C for 2 min and placed on ice, before the probe mix was pipetted into the hybridization chamber and hybridization was performed at 37C overnight.

Article Title: Tumor cell villages define the co-dependency of tumor and microenvironment in liver cancer
Article Snippet: The sections were then rinsed with 2× saline sodium citrate (SSC) for 5 min. NanoString ISH probes were prepared by incubation at 95 °C for 2 min and placed on ice, and the ISH probe mix (1 nM 980 plex ISH probe, 10 nM Attenuation probes, 1× Buffer R, 0.1 U/μL SUPERase•InTM [Thermofisher] in DEPC H 2 O) was pipetted on the tissue slides. .. An adhesive SecureSeal Hybridization Chamber (Grace Bio-Labs) was placed over the tissue. .. The hybridization chamber was sealed to prevent evaporation, and hybridization was performed at 37 °C for 16–18 h. Tissue sections were rinsed of excess probes in 2× SCT for 1 min and washed twice in 50% formamide (VWR) in 2× SSC at 37 °C for 25 min, then twice with 2× SSC for 2 min at room temperature.



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Grace Bio-Labs adhesive secureseal hybridization chamber
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